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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important job in advertising and marketing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two additional splice variants were being documented but in-depth nucleotide sequence information just isn't nevertheless obtainable.
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Blocking peptides are peptides that bind specifically on the goal antibody and block antibody binding. These peptide generally includes the epitope identified by the antibody.
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This manual outlines even more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
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This manual outlines more attainable optimization of mobile and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, you can see which staining is precise.
Focus on Data Cyclin E belongs on the very conserved cyclin read more loved ones, whose members are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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